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NF-E2-related factor 2 (Nrf2) is a master regulator of antioxidant defense system which can maintain the oxidation balance in the cell. In our previous study, we first cloned the Nrf2 gene in clams and preliminarily explored the role of the Nrf2 at the transcription level. In this study, RNA interference (RNAi) technology was used to interfere with the expression of Nrf2 after being exposed to benza)pyrene (BaP) for 5 days to verify the role of Nrf2 in the antioxidant defense system. Besides, we examined the mRNA expression and enzyme a