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The mutual regulation of AtR8 lncRNA and WRKY53/WRKY70 is mediated by Nonexpressor of Pathogenesis-Related Gene 1 (NPR1).Thermal stability, salt tolerance, and solubility in normal and high salinity brine are the major requirements for any surfactant designed for oilfield applications because the surfactant stays in a non-ambient environment inside the reservoir for a long period of time. Herein, a series of new gemini cationic surfactants (GSs) with varying spacer hydrophilicity were synthesized and elucidated using MALDI-ToF-MS, NMR (