https://www.selleckchem.com/pr....oducts/ins018-055-is
Using 'Duncan' grapefruit epicotyls as explants, 19 genome-edited grapefruit plants were generated with one biallelic mutant line (#dunyao7). Xcc caused canker symptoms on wild-type and non-biallelic mutant plants but not on #dunyao7. XccPthA4 mutant containing the designer TAL effector dLOB1.5, which recognizes a conserved sequence in both wild-type and #dunyao7, caused canker symptoms on both wild-type and #dunyao7. No off-target mutations were detected in #dunyao7. This study represents the first time that CRISPR-mediat