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99 and 1.63 in HFD/SED compared to CTRL/EX (RR = 1.99; 95% CI, 1.20-3.31; p = 0.0081) and CTRL/SED (RR = 1.63; 95% CI, 1.06-2.49; p = 0.025, respectively. The number of mice at endpoint with cancer was higher in HFD/SED compared to CTRL/EX and CTRL/SED (p 0.05). Healthspan index was highest in CTRL/EX (score = +2.5), followed by HFD/EX (score = +1), and HFD/SED (score = -1) relative to CTRL/SED. This work provides the basis for future preclinical studies investigating the dose-response relationship between exercise training and l