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The operational adaptability of our approach is showcased through the demonstration of its application in multiple bioanalytical scales. Within centimeter-scale ATPS studies, the use of PEG 6000 Da and dextran 10000 Da in a 10 mM PBS solution produced the optimized distribution ratios of 91682 for IgG and 0998 for FITC. Employing a millimeter-scale ATPS methodology, a 6067-fold amplification of analyte concentration is accomplished using a 15 wt% solution o