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Similar to previous reports detailing differential gene expression (DEGs) in embryonic stem cells (ES), our study found CCND1, HMCN1, and NKX2-2 to be among the most highly expressed genes; conversely, TWNC1, MYBPC1, and CKM were observed to be among the lowest expressed. GO, KEGG, and GSEA enrichment analyses revealed significant enrichment of differentially expressed genes (DEGs) associated with bone and muscle function, along with