https://www.selleckchem.com/pr....oducts/as2863619.htm
To study the proapoptotic effect of ligustilide on osteoblastoma (OS) and the relative related molecular mechanism. An MTT was used to examine the proliferation of OS cells, and Flow cytometry was used to analyze apoptosis and the cell cycle. Western blotting was used to detect the signaling pathway of apoptosis, and immunohistochemical (IH) staining was used to detect the apoptosis status of OS cells. A TLR4 inhibitor was used to study the effect of ligustilide on OS. Ligustilide inhibited OS cell proliferation but had no inhibitory
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