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tessellata. The study could help in understanding the adaptation mechanism of in vitro regenerated plantlets (especially velamen tissues of epiphytic roots) when transferred to the greenhouse for acclimatization. RESEARCH HIGHLIGHTS Vanda tessellata is an epiphytic orchid of horticultural importance. Comparative micro-morpho-anatomical analysis at subsequent stages of in vitro regeneration was conducted. Foliar structural and developmental mechanisms towards epiphytic adaptation were studied. In vitro induced structural abnormalities were