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In conclusion, the addition of honeybee pollen at a concentration of 1.0 μg/ml to IVM medium improved the in vitro maturation rate of sheep oocytes, increased the glutathione concentration, and improved gene expression.Silicon (Si) is a beneficial element which was proven to enhance the tolerance of plants to excess metal in a given growth medium. However, the efficacy of Si in mitigating Cu toxicity in plants can vary between plant species and with the amount of copper (Cu) present in the soil/medium. An experiment was performed to inv