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Increasing N dose further aggravated the severity of water deficit, decreased An, Gs and WUEi, damaged the structure and reduced the number of mitochondria of leaves, while increased Rd/An considerably under moderate water stress. Consequently, the biomass accumulation, carbon gain and plant level WUEp in the moderately water stressed treatment decreased markedly under the high N supply. Therefore, excessive N application should be avoided when plants suffer soil water stress in maize production.Low temperature in early spring severely