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For some samples, the SageELF program ‘0.75% agarose, 10 kb–40 kb’ (target 10,000 bp in properly 1 was used, and fractions 6 and seven have been pooled collectively for sequencing (average measurement of 17–21 kb). Samples were sequenced to a minimum HiFi knowledge quantity of 96 Gbp (30× estimated genome coverage) on at least 4 SMRT Cells. We next benchmarked variant calling utilizing parent–child trios. Furthermore, improvements appeared to be additive to these from the pangenome. The colour gradi