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The objective of this paper is to provide an estimation of air emissions (CO2, NOx, SOx and PM) released by port assistant vessels at port level. The methodology is based on the "full bottom-up" approach and starts by assessing the fuel consumed by each tug ship during its individual port exercises (movements during docking and undocking of merchant vessels). The scenario selected for the analysis and measurements is one of the most significant Port of the Mediterranean Sea, where seven auxiliary vessels were monitored for 407 calls. The