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Transcriptomic analysis of the Cu004 and Con groups identified 101 differentially expressed genes, of which 47 were upregulated and 54 were downregulated. Of note, the DEGs exhibited significant correlations with intestinal tissue formation, immune responses, apoptosis, and resistance to DNA damage and pathogen invasion. Chronic copper exposure's effects, as revealed by the findings, include intestinal histological modifications, the activation of antioxidant and immune processes, a
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