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Employing DNA's programmability, custom-designed static and dynamic nanostructures can be constructed; however, the assembly process, typically requiring high magnesium ion concentrations, often restricts their implementation. In the various solution conditions tested for assembling DNA nanostructures, the scope of suitable divalent and monovalent ions has so far been limited to examples like Mg2+ and Na+. DNA nanostructure assembly is investigated within a spectrum of ionic conditions, employing diverse-sized nanostructures