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The antiproliferative activity's assessment involved the use of an MTT assay. Evaluation of mRNA and protein expression in Notch signaling molecules was conducted through real-time PCR and Western blot. In HuCCT-1 cells, attractylodin and -eudesmol displayed a moderate inhibitory effect on cell growth, with corresponding IC50 values of 2900 ± 644 and 1680 ± 441 g/mL, respectively. The signaling molecules influenced the extent and direction of modulation in mRNA and protein expression within the CCA cell line. In the c
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