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Concurrent analysis of our data implies that a high dosage of AS could potentially curb Th1 and Th17-induced skin inflammation, while simultaneously encouraging the development of type 2-related skin inflammation. These results promise a deeper understanding of the skin's altered immune response to environmental factors following vaccination with an aluminum adjuvant. A casting technique was employed to prepare mucoadhesive films containing doxycycline hyclate (Doxy Hyc), comprising mixtures of hydroxypropylmethyl cellulose (HPMC) E3, K4 and polyacrylic