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001) which can be attributed to the significant decrease in apoptotic cells (P0.05) as evidenced by cell cycle analysis. Preconditioning of Piper longum L. (10-100μg/mL) enhanced Ca accumulation and matrix mineralization as observed by Von Kossa and Alizarin Red staining where ALP activity was elevated 3.6 folds as compared to untreated WJMSCs (P0.001). RT-PCR analysis exhibited up regulation of Runx2, Osterix, ALP and OPN mRNAs. We demonstrate for the first time that Piper longum L. fruit extract enhanced osteogenic differentiation of