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We delineate the calibration of flow, along with imaging C. elegans under fluorescence microscopy, to ascertain their molecular and/or cellular response to chemical sensory stimuli. Amphid neurons can also benefit from this protocol, which is facilitated by inserting the worm head-first into the chip. Detailed instructions for executing this protocol and its complete application are available in Bruggeman et al. (2022). Mycobacterium tuberculo
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