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Our study examined lipogenesis and the release of the pro-inflammatory cytokines IL-6 and IL-8 in vitro in SZ95 sebocytes after treatment with saturated fatty acids such as palmitic acid (C16:, margaric acid (C17:, and stearic acid (C18:, alone or in combination with selective inhibitors of SCD1 and FADS2 desaturases, and an agent with mixed inhibitory effects on FADS1 and FADS2 enzyme function. Through the combined action of SCD1 and FADS2 catalyzed pathways, C160 underw