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In contrast, multilocus sequence typing resolved 148 ticks into 39 genotypes of ticks and three genotypes of FLEs. The ST2-FLE genotype was carried by the tick genotype ST35, while the ST1-FLE genotype and 41.89% of the ST3-FLE genotype were carried by the tick genotype ST32. Accordingly, there appeared to be no specialization of certain tick genotypes to harbor-specific FLE genotypes. For the 1 time, we have provided an overview of the population structure of ticks and FLE strains. We found a low level of genetic diversity among FLEs