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A comparative analysis was performed to evaluate the agreement between mNGS and CMT, as well as mNGS's contribution to patient care. Evaluated in relation to clinical diagnosis, mNGS demonstrated a significantly superior overall PPA compared to CMT (531% [95% CI = 497 to 566%] versus 258% [95% CI = 228 to 289%]), while exhibiting a comparable NPA (932% [95% CI = 913 to 951%] versus 972% [95% CI = 959 to 984%]). In pediatric septic patients under six years of age, or those wit