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By means of co-immunoprecipitation and a mammalian two-hybrid assay, we ascertained that HCR lessened the activity of RAD51 interacting with BRC repeats, and substitutions at the phosphorylation sites on HCR influenced this decrease. From canine tumor samples, we ascertained ten mutations, one of which being a novel HCR mutation (I1110M) from canine tumor
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