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rse. Implementation of CSF NfL as a potential read-out for future therapeutic trials in ALS is currently constrained by its demonstrated susceptibility to (pre-)analytical variations. Here we show that the D50 model enables the discovery of correlations between clinical characteristics and CSF analytes and can be recommended for future studies evaluating potential biomarkers.Despite the success of Deep Neural Networks-a type of Artificial Neural Network (ANN)-in problem domains such as image recognition and speech processing, the energy and processing deman