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Pkapy and Ppapy, as determined by immunologic analysis of BALB/c mice, were found to be immunogenic, presenting with partial cross-reactive immune responses. The finding of a Th1-dominant immune response to Pkapy underscores its potential as a building block in an anti-Leishmania vaccine. Medium optimization represents a critical stage during cell culture, particularly in biopharmaceutics and regenerative medicine, but it