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An air mixing system for anaerobic digestion has been proved to be beneficial for methane production. The aim of the present study was to further investigate the appropriate conditions for air mixing. The effective methane production time (EMPT) was defined to determine the air mixing time in the article. The results indicated that the appropriate aeration intensity was 66.7 mL air per volume of reactor per min and mixing time was 1.5 min. When air mixing time exceeded 3 min on each occasion, total CH4 production was less than that achi