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A tube formation assay was performed to examine the influence of CLR extract on the in vitro process of angiogenesis. In vivo vascularization was determined using a zebrafish embryo model, which contained CLR extract. Phosphorylation status of VEGFR2, PI3K, AKT, and eNOS proteins was assessed by means of Western blotting. Additionally, the determination of nitric oxide (NO) and reactive oxygen species (ROS) levels was carried out independently using the Griess assay and the 2',