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© 2020 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.Determining factors that contribute to inter- and intra-individual variability in pharmacokinetics and drug metabolism is essential for the optimal use of drugs in humans. Intestinal microbes are important contributors to variability; however, such gut microbe - drug interactions and the clinical significance of these interactions are still being elucidated. Traditional pharmacokinetics can be complemented by untargeted mass spectrometry (MS) coupled with molecular networking to stu