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Bacterial panicle blight (BP caused by Burkholderia glumae is one of the most severe seed-borne bacterial diseases of rice in the world, which can decrease rice production by up to 75%. Nevertheless, there are few effective measures to manage this disease. In an attempt to develop an alternative management tool for BPB, we isolated and characterized phages from soil and water that are effective to lyse several strains of B. glumae. After tests of host ranges, the phages NBP1-1, NBP4-7 and NBP4-8 were selected for further comprehensive