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Employing the MTT assay, the antiproliferative activity was determined. The mRNA and protein expression of Notch signaling molecules were measured through real-time polymerase chain reaction and Western blotting procedures. The impact of attractylodin and -eudesmol on HuCCT-1 cell growth was moderate, as evidenced by IC50 values of 2900 ± 644 g/mL and 1680 ± 441 g/mL, respectively. The signaling molecules influenced the extent and directio