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Co-culture experiments with P. obtusidens and biofilm suspension revealed an inhibitory rate of 796 21%. The highest inhibitory rate was observed in H. akashiwo co-cultures with biofilm filtrate without nutrient replenishment, reaching 886 58%. The addition of nitrate and phosphate yielded a 723 20% inhibition rate for P. obtusidens, with no resulting inhibition observed within the H. akashiwo population. Interference competition was the primary mode of inhibition for P. obtusidens, whil
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