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Early metabolic bone disease (MBD) detection is important in preterm infants to decrease long-term consequence. We aim to explore the early MBD biochemical marker in extremely low-birth-weight (ELBW) infants. Retrospective cohort study of 95 preterm infants born in a tertiary care-level neonatal intensive care unit between January 2015 and June 2018, with birth weight 1000g. Thirty-five infants were "nothing by mouth" for 14days and categorized as the high-risk group; the remaining 60 were categorized as the control group. Minera