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25 g/L) and K2HPO4 (0.6 and 0.8 g/L) relative to normal culture conditions. Effects of the generated EPS under varying concentration of MgSO4 (r = 0.84-0.99) and K2HPO4 (r = 0.76-0.97) remained strongly correlated with cell count, chlorophyll content, total biomass, glucose, proliferative index and its scavenging activity. Collectively, our data not only showed that EPS generated by S. acutus under higher concentration of K2HPO4 and MgSO4 possess improved immunostimulatory properties, but also provides convincing evidence towards nutri