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The hypothetical nuclease TTHA1873, operating in a divalent metal ion solution, indiscriminately cuts methylated and unmethylated DNA, leading to plasmid DNA relaxation when facilitated by ATP. EDTA's chelation of metal ions leads to the inhibition of their activity. Further investigation into the hypothetical protein TTHA1873 is warranted by these results, which suggest a CRISPR-associated protein capable of non-specific DNase activity and ATP-dependent DNA unwinding. Ethylene oxide (EtO), despite