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From the key components of the original tumor – epithelial, vascular endothelial, fibroblast, and macrophage cells – organoids are developed. These are then enveloped with T cells to form an external layer, promoting T cell infiltration and the study of cytotoxicity. Within the context of a PDA organoid-based screen, epigenetic inhibitors ITF2357 and I-BET151 were discovered, and their combination with anti-PD-1-based therap