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Conclusion subjects with a stable weight and those who lost weight ≥ 5% from their maximum reported lifetime weight had significantly higher 25(OH)D concentrations than those who did not. Thus, maintaining over time may be an effective strategy to reach optimal serum 25(OH)D levels. 5 % from their maximum reported weight had significantly higher 25(OH)D levels than those who did not. Conclusion subjects with a stable weight and those who lost weight ≥ 5% from their maximum reported lifetime weight had significantly higher 25(OH)D concent