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Level III evidence is demonstrated by well-designed analytic studies, including cohort and case-control studies. Of all vertebrate groups facing extinction, amphibians hold the dubious distinction of first place, with freshwater fishes trailing in second. The preservation of freshwater fish species in developing nations is frequently obstructed due to the inadequate availability of information and data. The benchmark established by the International Union for Conservation of Nature (IUCN)'s Red List assessments for monitoring and mitigating species extinction
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