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© 2020 Ward et al.The plant vascular system has remained an under-explored niche despite its potential for hosting beneficial microbes. The aim of this work was to determine the origin of the microbial endophytes inhabiting grapevine. We focused on a single commercial vineyard in California over a two-year period and used an amplicon metagenomics approach to profile the bacterial (16S -V4) and fungal (ITS) communities of the microbiome across a continuum of six grapevine compartments; bulk soil, rhizosphere, root, cordon, cane a