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Three expressing and three non-expressing cell lines (opposite orientations) were created through TashAT2 transfection of bovine BoMac cells within this investigation. RNA-Seq experiments resulted in the discovery of genes exhibiting differential expression. The resulting dataset was scrutinized in light of differentially expressed genes identified in infected and non-infected cells, as well as in infected cell lines from susceptible Holstein and tolerant Sahiwal cattle. TashAT2's influence on bovine g