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The temperature effect on bioelectrochemical reduction of CO2 to acetate with a mixed-culture biocathode in the microbial electrosynthesis was explored. The results showed that maximum acetate amount of 525.84 ± 1.55 mg L-1 and fastest acetate formation of 49.21 ± 0.49 mg L-1 d-1 were obtained under mesophilic conditions. Electron recovery efficiency for CO2 reduction to acetate ranged from 14.50 ± 2.20% to 64.86 ± 2.20%, due to propionate, butyrate and H2 generation. Mesophilic conditions were demonstrated to be more favorable for biofil