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A nurse explores the unique grief of losing a child. Studying posttranslational modifications classically relies on experimental strategies that oversimplify the complex biosynthetic machineries of living cells. Protein glycosylation contributes to essential biological processes, but correlating glycan structure, underlying protein, and disease-relevant biosynthetic regulation is currently elusive. Here, we engineer living cells to tag glycans with editable chemical functionalities while providing information on biosynthesis,

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tro embryo production in Nili-Ravi buffaloes.In this study, it was found that monochloramine induced the formation of reactive species during ozonation of chloromethylisothiazolinone (CMIT). CMIT was found recalcitrant to chloramine. However, chloramine promoted the degradation of CMIT by ozonation significantly. Hydroxyl radicals contributed most to CMIT degradation (87%) during ozone/chloramine synergistic oxidation process (SOP). The hydroxyl radical exposure during ozone/chloramine SOP was around 7.9 times higher than that of ozonat

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Iron Dextran is an injectable low-molecular-weight ferric hydroxide complex indicated for patients with iron deficiency anemia where oral iron therapy is ineffective or poorly tolerated. It is particularly useful in iron deficiency resulting from excessive iron loss (e.g., hereditary hemorrhagic telangiectasia, excessive blood loss, etc.) or iron malabsorption (e.g., Crohn disease, celiac disease, inflammatory bowel disease, gastric bypass, etc.). Appropriate uses also include iron deficiency anemia resulting from menometrorrhagia, preg

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The largest ever Sri Lankan dengue outbreak of 2017 provides an opportunity for investigating the relative contributions of climatological, epidemiological and sociological drivers on the epidemic patterns of this clinically important vector-borne disease. To do so, we develop a climatologically driven disease transmission framework for dengue virus using spatially resolved temperature and precipitation data as well as the time-series susceptible-infected-recovered (SIR) model. From this framework, we first demonstrate that the distinct c

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Based on a suggested split into training and test sets and additional single-nuclei expert annotations, machine learning-based image segmentation methods can be trained and evaluated.It is only recently, with the advent of long-read sequencing technologies, that we are beginning to uncover previously uncharted regions of complex and inherently recursive plant genomes. To comprehensively study and exploit the genome of the neglected oilseed Brassica nigra, we generated two high-quality nanopore de novo genome assemblies. The N50 c

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Antibiotic exposure leads to massive selective pressures that initiate the emergence and spread of antibiotic resistance in commensal and pathogenic bacteria. The slow process of developing new antibiotics makes this approach counterintuitive for combatting the rapid emergence of new antibiotic resistant pathogens. Therefore, alternative approaches such as, the development of nucleic acid-based anti-bacterial treatments, anti-bacterial peptides, bacteriocins, anti-virulence compounds and bacteriophage therapies should be exploited to c

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Differences were considered significant at P less then 0.05. The results were that the FA value in the LITUS treatment group at 1 day after TBI was significantly higher than that in the control group (adjusted P = 0.0422) and significantly lower than that in the TBI group at 14, 21, and 35 days after TBI (adjusted P = 0.0015, 0.0064, and 0.0173, respectively). At the end of the scan time point, the differences between the two groups were not significant (adjusted P = 0.3242). The MD values in the LITUS treatment group we

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Polycyclic endoperoxides are rare natural metabolites found and isolated in plants, fungi, and marine invertebrates. The purpose of this review is a comparative analysis of the pharmacological potential of these natural products. According to PASS (Prediction of Activity Spectra for Substances) estimates, they are more likely to exhibit antiprotozoal and antitumor properties. Some of them are now widely used in clinical medicine. All polycyclic endoperoxides presented in this article demonstrate antiprotozoal activity and can be divid

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ures determined under acidic conditions provide insight into capsid changes associated with endosomal and gastrointestinal acidification. Structural rearrangements of the capsid VP N-terminus, at the base of the 5-fold channel, demonstrate a disordering of a "basket" motif as pH decreases. These observations begin to unravel the molecular mechanism of HBoV infection and provide information for control strategies.Tembusu Virus (TMUV) is an emerging and re-emerging zoonotic pathogen that adversely affects poultry industry