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Compared to other respiratory viruses, coronavirus infections revealed a unique dysregulation of the N-degron pathway. The SARS-CoV-2 infection process triggered a rise in ATE1 expression levels discernibly in Calu-3 and Vero CCL-81 cell cultures. Nevertheless, the infection of macrophages did not alter the enzyme regulatory processes. ATE1 protein arginylation, ascertained using P1 and P2 viral variants and HEK 293T