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Moisture content is an important index to evaluate the water content in substrate. Near-infrared (NIR) spectroscopy was used for rapid quantitative detection of moisture content of coco-peat substrate. The different spectral pretreatment methods were adopted to pre-process the spectral data. Successive projection algorithm (SPA), elimination of uninformative variables algorithm (UVE) and synergy interval partial least squares algorithm (Si-PLS) were used to screen characteristic variables of coco-peat substrate original spectral data and