https://www.selleckchem.com/pr....oducts/Rapamycin.htm
Objective To investigate whether CD137 signaling can promote angiogenesis via regulating macrophage M1/M2 polarization. Methods (1) The primary peritoneal macrophages in mice induced by 3% thiglycollate broth were divided into three groups control group, CD137 signaling activated group and CD137 signaling inhibited group. Various specific markers of M1 and M2 macrophages were detected to observe the phenotype change of macrophages, and the macrophages protein expression of CD137, CD86 and CD206 was detected by flow cytometry (FCM). Th