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In co-cultures of P. obtusidens with biofilm suspensions, the highest observed inhibitory rate was 796 21%. Conversely, co-cultures of H. akashiwo with biofilm filtrate (without nutrient addition) yielded an inhibitory rate of 886 58%, which was the highest. Upon the introduction of nitrate and phosphate, the P. obtusidens inhibition rate reached 723 20%, yet no such impact was discernible on the H. akashiwo population. Interference competition played a significant role in the i