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Bisphenol A (BPA) is an endocrine-disrupting chemical. Its influence on lipid homeostasis remains to be proven. In this study, the obese model of laying hens were induced using high-fat diet (HFD) to determine the lipid metabolism interference of BPA, especially its influence on estrogen receptors (ERs) and oxidative damage, at the dose of tolerable daily intake (TDI, 50 μg/kg body weight [BW]/day) and no observable adverse effect level (NOAEL, 5000 μg/kg BW/day). The results demonstrated that the TDI dose of BPA interacted with ERα mo