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Patients who have undergone transsphenoidal surgery (TSS) encounter perioperative hormonal variations, but there is little research on perioperative serum and hair steroid profile modifications. Changes in steroid metabolic profiles were studied in patients with nonfunctioning pituitary adenomas (NFPA) who underwent transsphenoidal surgery (TSS) perioperatively. Enrollment included 55 participants who underwent trans-sphenoidal surger