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We utilize microfluidic approaches to test the hypothesis that introducing Laponite nanoclay into collagen hydrogels changes their mechanical and rheological characteristics, and that adjusting the stiffness of the collagen microenvironment impacts tumor spheroid expansion. Our research corroborates our hypotheses, indicating that ECM components and engineered tumor models are beneficial for cancer research, providing a biocompatible and biomimetic approach to adjusting the mechanical properties of st