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Microbial community dynamics and PTA wastewater degradation performance of sequentially connected two-stage upflow anaerobic sludge blanket (UAS bioreactors have been studied for 225 days. The working volume of acidogenic (R1) and methanogenic reactors (R2) have sixfold differences. Thus, the reactors operated under different hydraulic retention time (HRT) conditions, which are preferential for PTA wastewater content. Archeal and bacterial profiles of granules were analyzed with denaturing gradient gel electrophoresis (DGGE) and real-t