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Specifically targeting LC1 and LC2 tags, we integrated them with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated proteins (Cas12a) system and pre-amplification using recombinase polymerase amplification (RPA). Our analysis of the dual-target RPA-CRISPR/Cas12a assay revealed a sensitivity level of roughly 0.2 copi