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Synthetic pharmaceuticals are deemed less desirable than herbal preparations, and the employment of time-honored medicinal practices is expanding. The current investigation into the potential bioactive compounds of Citrullus colocynthis utilized FTIR, HPLC, and GC-MS analyses, highlighting the presence of secondary metabolites at notable concentrations—including gallic acid (74854 ppm), vanillic acid (122616 ppm), and ferulic acid (101045 ppm)—exhibiting considerable bioactivities. The antimicrobial protein content of each protein fraction was measured th
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